How to Make a Still Air Box: DIY Guide

Spread the love
How to Make a Still Air Box

How to Make a Still Air Box

How to Make a Still Air Box: A still air box is an essential tool for microbiological work, providing a controlled environment to prevent contamination during delicate procedures. This article presents a step-by-step guide on how to construct a still air box for your microbiological experiments. By following these instructions, you can create a cost-effective and efficient solution to safeguard your work from unwanted contaminants.

Materials Needed:

  1. Work Surface:
    • A sturdy table or bench to serve as the foundation for your still air box.
  2. Enclosure:
    • A clear acrylic or polycarbonate box with dimensions suitable for your workspace.
    • Ensure one side is easily removable for access to the interior.
  3. Gloves:
    • Latex or nitrile gloves to maintain aseptic conditions.
  4. Air Filtration System:
    • HEPA (High-Efficiency Particulate Air) filter with a fan.
    • A small, quiet computer fan is suitable for this purpose.
  5. Light Source:
    • LED light strip or battery-powered LED lamp to illuminate the interior.
  6. Sealant:
    • Silicone sealant for attaching the fan and creating airtight seals.
  7. Tools:
    • Screwdriver
    • Drill
    • Hole saw or jigsaw
    • Measuring tape
    • Sandpaper

Construction Steps:

  1. Measure and Mark:
    • Measure the dimensions of your enclosure and mark the areas where the fan, gloves, and access panel will be installed.
  2. Cut Openings:
    • Use a hole saw or jigsaw to cut openings for the fan on one side of the box. Ensure a snug fit for the fan.
    • Cut holes for gloves on either side of the box.
    • If not supplied with pre-cut openings, create a removable access panel on one side of the box.
  3. Install Gloves:
    • Attach the gloves to the holes using a suitable sealant. Ensure a tight, airtight seal.
  4. Mount the Fan:
    • Securely attach the fan to the designated opening using screws.
    • Position the fan to draw air through the HEPA filter and into the box.
  5. Attach the HEPA Filter:
    • Place the HEPA filter in a way that allows the fan to draw air through it.
    • Seal the edges of the filter to prevent any air bypass.
  6. Create the Access Panel:
    • If not pre-existing, attach a clear, removable panel to allow access to the interior of the box.
  7. Seal Joints:
    • Apply silicone sealant to all joints, ensuring the box is airtight. Pay special attention to the edges of the gloves, fan, and access panel.
  8. Attach Lighting:
    • If desired, attach the LED light strip or battery-powered LED lamp to the interior of the box for better visibility.
  9. Test for Airtightness:
    • Before use, conduct a thorough check to ensure the box is airtight. Any leaks should be sealed with additional silicone.
How to Make a Still Air Box


By following these step-by-step instructions, you can build your own still air box for microbiological work. This DIY solution offers a cost-effective alternative to commercially available options, allowing you to conduct your experiments in a controlled and contamination-free environment. Always prioritize safety and cleanliness throughout the construction process to guarantee the success of your microbiological work.

Read also:

How to Clean Your Igloo Ice Maker for Optimal Performance

How to Deal with Ungrateful Stepchildren

How to Cancel an Order on Amazon: A Step-by-Step Guide

Rate this post

Leave a Comment

Your email address will not be published. Required fields are marked *

Scroll to Top