How to Make a Still Air Box: DIY Guide

How to Make a Still Air Box

How to Make a Still Air Box: A still air box is an essential tool for microbiological work, providing a controlled environment to prevent contamination during delicate procedures. This article presents a step-by-step guide on how to construct a still air box for your microbiological experiments. By following these instructions, you can create a cost-effective and efficient solution to safeguard your work from unwanted contaminants.

Materials Needed:

1. Work Surface:
   • A sturdy table or bench to serve as the foundation for your still air box.
2. Enclosure:
   • A clear acrylic or polycarbonate box with dimensions suitable for your workspace.
   • Ensure one side is easily removable for access to the interior.
3. Gloves:
   • Latex or nitrile gloves to maintain aseptic conditions.
4. Air Filtration System:
   • HEPA (High-Efficiency Particulate Air) filter with a fan.
   • A small, quiet computer fan is suitable for this purpose.
5. Light Source:
   • LED light strip or battery-powered LED lamp to illuminate the interior.
6. Sealant:
   • Silicone sealant for attaching the fan and creating airtight seals.
7. Tools:
   • Screwdriver
   • Drill
   • Hole saw or jigsaw
   • Measuring tape
   • Sandpaper

Construction Steps:

1. Measure and Mark:
   • Measure the dimensions of your enclosure and mark the areas where the fan, gloves, and access panel will be installed.
2. Cut Openings:
   • Use a hole saw or jigsaw to cut openings for the fan on one side of the box. Ensure a snug fit for the fan.
   • Cut holes for gloves on either side of the box.
   • If not supplied with pre-cut openings, create a removable access panel on one side of the box.
3. Install Gloves:
   • Attach the gloves to the holes using a suitable sealant. Ensure a tight, airtight seal.
4. Mount the Fan:
   • Securely attach the fan to the designated opening using screws.
   • Position the fan to draw air through the HEPA filter and into the box.
5. Attach the HEPA Filter:
   • Place the HEPA filter in a way that allows the fan to draw air through it.
   • Seal the edges of the filter to prevent any air bypass.
6. Create the Access Panel:
   • If not pre-existing, attach a clear, removable panel to allow access to the interior of the box.
7. Seal Joints:
   • Apply silicone sealant to all joints, ensuring the box is airtight. Pay special attention to the edges of the gloves, fan, and access panel.
8. Attach Lighting:
   • If desired, attach the LED light strip or battery-powered LED lamp to the interior of the box for better visibility.
9. Test for Airtightness:
   • Before use, conduct a thorough check to ensure the box is airtight. Any leaks should be sealed with additional silicone.

Conclusion:

By following these step-by-step instructions, you can build your own still air box for microbiological work. This DIY solution offers a cost-effective alternative to commercially available options, allowing you to conduct your experiments in a controlled and contamination-free environment. Always prioritize safety and cleanliness throughout the construction process to guarantee the success of your microbiological work.

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